货号K1622 RevertAid First Strand cDNA Synthesis Kit 100 reactions
K1622逆转录酶试剂盒 Thermo反转录试剂盒
货号 单位规格 目录价格 (CNY)
K1622 100 reactions 1,951.00
K1622逆转录酶试剂盒 Thermo反转录试剂盒
Thermo Scientific RevertAid*链cDNA合成试剂盒包含了足够进行100次x20 μL反应的试剂。本品是以RNA为模板高效合成*链cDNA的完整系统，可合成长达13kb的cDNA。本品含有RiboLock RNase抑制剂，能够有效保护RNA模板不受降解。
Thermo Scientific RevertAid*链cDNA合成试剂盒配套提供oligo(dT)18引物和随机六聚体引物。oligo(dT)18引物可以选择性地与mRNA中的poly (A)尾结合。随机六聚体引物无需poly (A)尾，因此可转录mRNA 5’-端区域，也可以无poly (A)尾的RNA (如micoRNA)为模板合成cDNA。该试剂盒也可使用基因特异性引物。
特点：
• 可以高效合成长达13kb的全长*链cDNA
• *活性温度为42°C
• *一提供RT反应所需的所有组分
应用
• *链cDNA合成，作为RT-PCR和实时RT-qPCR的模板
• 构建全长cDNA文库
• 反义RNA合成
For Research Use Only. Not for use in diagnostic procedures.
K1622逆转录酶试剂盒 Thermo反转录试剂盒
| Reverse Transcriptase: | RevertAid Reverse Transcriptase |
|---|---|
| Downstream Application: | Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR) |
| Reaction Format: | Separate Components |
| Sample Type (General): | RNA |
| Product Size: | 100 reactions |
| Optimal Reaction Temperature: | 42° C |
The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
Store at -20°C.
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