甘氨酸

Analysis Note

经过检测，可用于 PAGE 电极缓冲液和蛋白质印迹转移缓冲液。

Application

甘氨酸是用于聚丙烯酰胺凝胶电泳的 Tris-甘氨酸和 Tris-甘氨酸-SDS 运行缓冲液的成分¹。甘氨酸也是蛋白质印迹 Towbin 转移缓冲液的成分²。

Biochem/physiol Actions

脊髓中的抑制性神经递质，NMDA 受体的变构调节剂。

1. Hames, B.D. and Rickwood, D. Gel Electrophoresis of Proteins: A Practical Approach 2nd ed., New York, NY , (1990), 32-35

2. Towbin, H., et al., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U. S. A. 76, 4350, (1979)

Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970)

Logue, S.E., et al., Expression, purification and use of recombinant annexin V for the detection of apoptotic cells. Nat. Protoc. 4, 1383-95, (2009)

Smith, K., et al., Rapid Generation Of Fully Human Monoclonal Antibodies Specific To A Vaccinating Antigen. Nat. Protoc. 4, 372-84, (2009)

Yu, D., et al., Fractionation Of Different PEGylated Forms Of A Protein By Chromatography Using Environment-responsive Membranes. J. Chromatogr. A. 1217, 5595-601, (2010)

Rosner, M., et al., Efficient SiRNA-mediated Prolonged Gene Silencing In Human Amniotic Fluid Stem Cells. Nat. Protoc. 5, 1081-95, (2010)

Røtterud, R., et al., The Star Chart To Ta Bladder Cancer: An Unsophisticated Analysis Of Two-dimensional Gel Electrophoresis Proteome Maps. Scand. J. Urol. Nephrol. 44, 76-83, (2010)